I have gone through literature but I am confused about what is a basic protocol for fatty acid profiling from rice seeds. Is it to first extract lipid and then convert it to Fatty acid methyl esters ( FAME)? Because some protocol includes direct conversion to FAME.? Some protocol involve TLC followed by GC.
So which is protocol is better?
Most modern lipidomics workflows depend on LC-MS, although sometimes shotgun (no chromatographic separation) is preferred. FAME analysis on the other hand, has predominantly been conducted using GC-MS and it seems as if GC-MS still has some advantages over LC-MS for (low abundant) fatty acid analysis [1]. Generally, however, we can also identify fatty acids in LC-MS data. It really depends on where your focus lies.
[1] Chiu HH, Kuo CH. Gas chromatography-mass spectrometry-based analytical strategies for fatty acid analysis in biological samples. J Food Drug Anal. 2020;28(1):60-73. doi: 10.1016/j.jfda.
https://www.sciencedirect.com/science/article/abs/pii/037843479500116Z
check this out
Hi
For fatty acid analysis you can use the follwoing paper.
https://doi.org/10.1007/s12161-021-02203-0
I hope this helps!
Hi
The calculation and processing protocols are well explained in the following paper
https://doi.org/10.1007/s12161-021-02203-0
I hope this helps!
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