Kajol B M Singh

4 Questions 4 Answers 0 Followers

Questions related from Kajol B M Singh

How to get bed12 format file of rice reference genome so as to use it in genomation package of R?

For annotation of Differentially methylated regions using genomation package. I require bed12 file. For that I used MSU rice gff3 file and converted it to bed format using bedtools. When I am...

03 December 2020 7,453 1 View

Is it possible after RNA seq to have only overlapping upregulated and overlapping downregulated genes between overexpression and rnai mutant samples?

RNA seq was done for wild type, overexpression and rnai mutant plants. After analysing the reads, it was found there are no common genes which is upregulated in ox plant and downregulated in rnai...

03 December 2020 5,683 1 View

How to get only my his tag protein purified using ni-nta ?

I want to purify a fragment of my interest gene. For that I have cloned my fragment into pET28a vector with his tag at its N-terminal, and transformed into both rosetta and BL21 strains. On...

23 November 2020 7,534 13 View

Is lipid profiling method different from fatty acid profiling?

I have gone through literature but I am confused about what is a basic protocol for fatty acid profiling from rice seeds. Is it to first extract lipid and then convert it to Fatty acid methyl...

01 January 1970 9,123 4 View