Some of researchers have used dried plant leaves for this purpose based on Bates's Method.
I think yes.
Please have a look at this useful ResearchGate link.
https://www.researchgate.net/post/Can_we_use_dried_leaf_sample_for_quantification_of_proline#post-comment-editor
Please also have a look at this RG link.
https://www.researchgate.net/post/What_is_the_simplest_method_to_detect_Proline_content#post-comment-editor
Yes, because correct drying only removes water :)
You should freeze-dry samples and it should work. Obviously you cannot dry your leaves by heating.
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