Is it safe to design primers from UTR regions to amplify the CDS?

Mahbobeh zamani babgohari @Mahbobeh-Zamani-Babgohari

31 January 2020 0 2K Report

I am cloning terpene synthase genes (CDS) there are different types of them in the same plant which can be quite similar too (i.e in some cases about 97% identity). I was wondering if I it would be recommended to design primers from UTR regions? Or I may end up with nothing to get amplified?

Thanks in advance for your suggestion,

Mah

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