I am analyzing absorbance of my monomer first I stuck in which kind of solvent I should use for the monomer and how much percentage of solute and solvent I should use for both of them (i.e 0.1 ml in 5 ml?)
And which reference I should use?
Hi Muhammad,
Find good solvents first. And measure absorbances of your compounds in various concentrations and calculate the molar extinction coefficients. if they are independent of the concentrations, your compound should be monomer in the solvent.
If your compound is having absorption maxima above 245 you can use chloroform but if it is absorbing around this advisable to try methanol of suitable purity grade.
Chloroform is best avoided because of its toxicity and environmental effects. However, it may be that your sample won't dissolve in anything much else. My first choice as an alternative is ethyl acetate, which has a similar degree of polarity to chloroform; it is also less restrictive in the UV, but may not be a good enough solvent for your substance. Methanol, as has already been suggested, is another good possibility; propan-2-ol is another.
We'd need to know more about your sample to be able to advise!
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