I am following the protocol (Pan et al 2010, http://www.nature.com/nprot/journal/v5/n6/full/nprot.2010.37.html) for the plant hormone extraction.
In the step 8, it is mentioned to concentrate the solvent mixture (not completely dry) using a nitrogen evaporator with nitrogen flow.
I was wondering if it is ok to use a vacuum evaporator instead of nitrogen evaporator?
and what would be the possible impact of complete drying of the solvent mixture?
I always extract the auxin I AA and ABA using 80% methanol and then evaporate under vacuum using cooled water bath attached to the rotary evaporator. i extract gibberellins using 80% acetone similarly. during the evaporation, the extract is evaporated to an aqueous phase which then made up to 50 ml with distilled water. then the extract are cleaned and shaken out with either freshly distilled ether for IAA,ABA , or ethyl acetate for gibberellins, then HPLC.
Hi!
You could do it but be carefull. If you evaporate minimal quantity of organic solvents (some ml) and they dont contact with mineral oil in equipment, it is OK. In other can they could dissolve oil and dstroy the system.
Dear Muayed Fadhil Abbas
In your answer you have mentioned "made up to 50 ml with distilled water"?
How much is your starting material? I am extracting from rice and arabidopsis leaves (50-100 mg).
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