I am working with a plasmid called pSIP 411. I have tried several methods of transforming this plasmid DNA in to L. reuteri like high salt, glycine enriched, PEG, sucrose, high voltage etc. I certainly fail!
L. reuteri grows well in aerobic/ micro-aerobic conditions. Is it necessary to grow them anaerobically during the incubation of plates after electric shock or during competent cell preparation?
Is there any other method to transform Plasmid in to L. reuteri?
The latest thesis attached here says "Despite our efforts our L. reuteri strain remained reluctant to any transformation protocol which might be due to its pronounced agglutination and therefore inaccessible cell wall."
How can I overcome this agglutination problem?
We have many of the latest papers that have used electroporation in L. reuteri. How do they do this? Any ideas, please let me know.
Were you able to resolve this issue? I also have been having trouble transforming a similar plasmid into L. reuteri.
I have a similar problem. If you have any information to overcome trouble, could you share with me? Thank you.
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