How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Is there any method that we can electrochemically oxidize a powder?

I am working with a material which is sensible to water and oxygen, I would like to oxidize this powder either chemical or electromagnetically. Does anyone knows a method to electrochemically...

09 February 2021 2,944 3 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

How to make sure each cavity modes with same E-field distribution in COMSOL simulation?

In COMSOL Application Library, there is case about waveguide adapter. But there are some questions: 1) In boundary mode analysis, it just set to find only 1 mode and the S-parameter is...

12 January 2021 9,383 2 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

Why the treatment days for the xenograft model is less than the observation days?

In some experiments for investigate drug effects for in vivo xenograft model, some authors onle give treatment to the mice for 14 days or 21 days, but the data collection/observation (such as...

13 December 2020 525 2 View

Dear professors, i am confused with how to figure out the tea bag index?

Hello, i am confused with how to figure out the tea bag index,could you please tell me how to compute the letter "a" and "k". Can i use R or Sigmaplot to fix it?

09 December 2020 6,705 3 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

How to use the bioluminescent ATP assay kit?

I need to do a mitochondrial ATP quantification assay in insects and for this, I have a bioluminescent ATP assay kit but I don't have a protocol yet. I'd like to know if you have some kind of...

24 January 2021 3,655 3 View

Can Vibrio campbellii bioluminescence be obtained when cultured on Mueller-Hinton agar?

Need to check quorum sensing activity of Vibrio campbellii.

20 January 2021 570 5 View

Luciferase assay without luminometer?

Hi everyone. I intend to carry out a luciferase assay for my research but have no access to a luminometer. Is there an alternative assay that I can use for my research or can anyone suggest a way...

05 December 2020 9,749 2 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

23 November 2020 2,813 2 View

Does anyone have experience in TOPFLASH assay in HEK293T cells?

I did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented with 10uM...

20 October 2020 3,584 2 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

15 October 2020 7,641 4 View

Will Cypridina Luciferase (CLuc) Catalyze Beetle Luciferin?

Hello, I am using a lentivirus encoding CLuc to transduce target cells. I want to then lyse the cells in the presence of luciferin and measure luminescence. The kit which I had hoped to use...

15 September 2020 6,664 2 View