Is it possible to use the boiling method for genomic extraction in gram positive bacteria (Staphylococcus species)?

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Kaushik Bhattacharjee

Hi Abdul, if you want only to identify these using molbio techniques, then you can opt for colony PCR (16S rRNA). Best of luck.

Syed Riyaz-Ul-Hassan

Yes, PCR templates can be prepared successfully from Staphylococcus spp. by this method. I am sending you my publication for details.

Here is the link:

http://www.sciencedirect.com/science/article/pii/S0740002008000269

Abdul Rahim

Ok...thank for the helping

Zina Hasan

Steve Shost

What is work of the Triton/TE mixture during DNA extraction of Staph. aureus by using boiling method?