How to analyse dru sequence of Staphylococcus species for dru typing?

Hi everyone..i just want to ask..how to analyse raw dru sequence of Staphylococcus species for dru typing identification of Staphylococcus species?..I already read several journals and they...

01 February 2016 9,082 3 View

How and how long can I preserve DNA extracted by boiling?

Hello and Good day everyone. Previously, I did DNA extraction from gram positive bacteria by boiling method. After that, I preserved it at temperature -20 degree which is very suitable for DNA...

03 April 2015 3,380 10 View

Is it suitable to used boiling method to do plasmid extraction from gram positive bacteria (staphylococcus species)?

Hi and Good day.. I just want to ask...i deal with staphylococcus species..so, i want to apply boiling method to extract the plasmid because previously i saw some researcher can get plasmid from...

02 March 2015 9,947 2 View

Can tyrosine intrinsic fluorescence be detected at an emission wavelength either than at 307nm?

11 December 2014 3,725 1 View

Isn't any difference in phenol concentration between fresh dissolve phenol with long time dissolve phenol?

I'm deal with phenol, i dissolve phenol with distilled water with certain concentration. I prepared this phenol solution around one or two month ago...but i'm doubtful about its concentration now...

11 December 2014 8,072 2 View

Can anyone recommend a method to find the active site of certain enzyme in bacteria?

For information, I'm work with an enzyme from bacteria. Unfortunately, almost all previous reports only discuss about the active site of this enzyme in yeast. I already tried to search information...

09 October 2014 557 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View