Abdul Rahim

7 Questions 7 Answers 0 Followers

Questions related from Abdul Rahim

How to analyse dru sequence of Staphylococcus species for dru typing?

Hi everyone..i just want to ask..how to analyse raw dru sequence of Staphylococcus species for dru typing identification of Staphylococcus species?..I already read several journals and they...

02 February 2016 9,135 3 View

How and how long can I preserve DNA extracted by boiling?

Hello and Good day everyone. Previously, I did DNA extraction from gram positive bacteria by boiling method. After that, I preserved it at temperature -20 degree which is very suitable for DNA...

04 April 2015 3,434 10 View

Is it possible to use the boiling method for genomic extraction in gram positive bacteria (Staphylococcus species)?

I want to do PCR screening for my staphylococcus species. So, i need genomic from that. Instead by using genomic kit, is it possible to used boiling method to lyse the cell to get the genomic. If...

03 March 2015 9,391 5 View

Is it suitable to used boiling method to do plasmid extraction from gram positive bacteria (staphylococcus species)?

Hi and Good day.. I just want to ask...i deal with staphylococcus species..so, i want to apply boiling method to extract the plasmid because previously i saw some researcher can get plasmid from...

03 March 2015 9,988 2 View

Isn't any difference in phenol concentration between fresh dissolve phenol with long time dissolve phenol?

I'm deal with phenol, i dissolve phenol with distilled water with certain concentration. I prepared this phenol solution around one or two month ago...but i'm doubtful about its concentration now...

12 December 2014 8,118 2 View

Can tyrosine intrinsic fluorescence be detected at an emission wavelength either than at 307nm?

I'm doing fluorescence spectroscopy on my protein expecting to see the intrinsic fluorescence of tyrosine and also its effect with addition of cu(i). The excitation wavelength was 280 nm while the...

12 December 2014 3,773 1 View

Can anyone recommend a method to find the active site of certain enzyme in bacteria?

For information, I'm work with an enzyme from bacteria. Unfortunately, almost all previous reports only discuss about the active site of this enzyme in yeast. I already tried to search information...

10 October 2014 611 6 View