I have to study PL of the polyimides in solution form and thin films spin coated on glass surface. Can I use same standard for both? Moreover, is there any relation between selection of standard and region of emitted light?
You could use the same standard for both, yet, to be safe, you may prepare more than one thin films having different thicknesses by altering the spin-coating speeds.
No problem, until you are getting considerable thickness of the film to get a reliable emission.
The region of emitted light is not much dependent on standard solution, obviously standard means not too thin or not too concentrated. In thinner solution, intensity is low and noise would contribute relatively greater part, in concentrated solution the intermolecular distance becomes low which changes and quenches intensity of emitted light.
Dear, You can use same standard for both without any problem and for obtaining on a different thin films you can control by the values of solution before coating or by change the speed of spin coating device.