Is it possible to use rat anti-mouse instead of mouse anti-rat on FACS assay?

More Moustafa Nouh Elemeery's questions See All

Anyone Knows How to perform stARTS analysis for lead molecule interaction fitness?

I wanna find a help, illustration or links for stARTS analysis for lead molecule interaction analysis validation against DICER and Drosha specially during Pre-miRNA and miRNA synthesis

11 December 2018 5,184 0 View

Can I use Trypan blue or anyother stain instead of Nitrotetrazolium Blue chloride in Anchorage independent technique assay?

in last step of anchorage independent assay, we required to use Nitrotetrazolium Blue chloride to stain the colonies so can we use any cheap and easy to use stain other than Nitrotetrazolium...

06 July 2018 9,191 1 View

Is there avaliability to get accessibility to clinical data like blood screening, Biological marker for colorectal cancer patients?

I tried many database but i cannot find biological marker and CBC, So if anyone know a website for that database or how to extracte from TCGA or other please refer to steps many thanks

11 December 2017 7,798 2 View

In miRNA study i found 2 median vlues ( spot median and bg median) what is the difference between each?

i need the basic diffence when using as a comparative values

11 December 2017 1,950 0 View

How can I predict, miRNA-miRNA interaction, if I have a specific panel of miRNAs?

I have a panel of miRNA that validated to be affected by expression of a targeted protein and another panel that in addition varied upon treatment, What I want is to find the miRNA-miRNA...

01 January 1970 3,723 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Iman Hassan Ibrahim

Species cross reactivity is not that common, I guess you need to try that on few samples first.

Kawther K. Ahmed

Hello,

you might end up under estimating your population of interest due to low antibody reactivity. As suggested above, the best way to decide is by running a small experiment comparing both antibodies at different concentration of both and compare you results.