in last step of anchorage independent assay, we required to use
Nitrotetrazolium Blue chloride to stain the colonies
so can we use any cheap and easy to use stain other than
Nitrotetrazolium Blue chloride
Hi Moustafa, as far as I can tell both stains are commonly used to assess cell viability.
Also both dyes are cell impermeable.
I wanna find a help, illustration or links for stARTS analysis for lead molecule interaction analysis validation against DICER and Drosha specially during Pre-miRNA and miRNA synthesis
11 December 2018 5,281 0 View
I tried many database but i cannot find biological marker and CBC, So if anyone know a website for that database or how to extracte from TCGA or other please refer to steps many thanks
11 December 2017 7,880 2 View
i need the basic diffence when using as a comparative values
11 December 2017 2,041 0 View
Hello is it possible to use CD8 (BV 786 rat anti-mouse) instead of CD8 (BV 786 mouse anti-rat), on FACS assay on rat whole spleen cells?
01 January 1970 7,276 2 View
I have a panel of miRNA that validated to be affected by expression of a targeted protein and another panel that in addition varied upon treatment, What I want is to find the miRNA-miRNA...
01 January 1970 3,799 5 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...
03 August 2024 5,384 0 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View