Is it possible to describe your personal clinical case or observation in review paper? In what part of the article should it be described?

There is an interesting clinical case and operative technique I am planning to describe. However, this technique was described previously with slight differences. Is it possible to write a review...

30 March 2020 5,710 3 View

Dynamic characteristics of the soil in HS small model (Plaxis)?

Dear all. To determine the stress-strain state of the construction-base system for seismic effects, is it possible to find the dynamic characteristics of the soil in HS small model (Plaxis) and...

09 February 2020 5,211 3 View

Problem purifying His-tag protein with Ni-NTA agarose?

Hello, I am trying to purify His-tag protein by affinity chromatography with Ni-NTA agarose but I am not able to elute it. I already purified recombinant protein with the same protocol few times...

17 December 2019 7,724 13 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

Small displacement measurement in vacuum?

Hello, I have a question. there is a circular thin foil which vibrates in vacuum, the foil restricted and moves up and down. it is like a diaphragm between two pipes . both sides are in vacuum but...

18 February 2021 8,075 3 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View