How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

How to amplify specific pcr product?

Hello I try to amplify gene of interest by RACE-PCR. For 5` RACE, I saw multiple bands after using specific nested primer. But The desired band was not seen on the gel. What is the reason? When I...

31 October 2020 7,734 6 View

What is the best force field for proteins with folded and disordered regions?

Hello I want to do MD simulation on a protein model to refine its structure. My protein structure have both folded and intrinsically disordered regions. What force field should i choose for this...

23 August 2020 9,381 5 View

What is the best force field for protein MD simulation?

Hello I try to do molecular dynamic simulation with GROMACS software and I work on an enzyme in a plant producing a medicinal secondary metabolite. When i was searching to find a appropriate force...

31 July 2020 171 5 View

How to avoid smear from PCR product on agarose gel?

Hello I used high fidelity enzyme in my PCR and I saw smear on agarose gel. I repeated the PCR with different amounts of cDNA. But I did`nt get a sharp band. what should i do to resolve my problem?

29 July 2020 3,483 10 View

Which one is more important in protein homology modeling ?

I try to make a model with MODELLER software and I decide to use multiple template modeling method. Two template structure I choose for my work, have similar Identity percentage (42.54% and...

03 May 2020 1,263 3 View

I am interested to know how can I modeling Troposphere with Bernese software? It means that can you introduce a source for it?

I readed the Bernese manual which that published at Bernese website, but it was'nt enough.

10 March 2020 6,098 3 View

How can i detect small size PCR product from primer dimer?

Do you know any method to distinguish small target band of primer dimer? ( They have similar size.) I tried higher concentration of agarose gel and longer electrophoresis run time. but i didn`t...

22 December 2019 387 9 View

Is it possible to estimate the most recent common ancester using o & 1 data? If yes would you please introduce a software?

Dear professors Respectfully, i know that sequences can help us to reveal the most common ancestor in phylogenetic studies. However, in genetic diversity studies using IRAP, SSR, markers etc, i...

13 November 2019 9,305 6 View

Have you ever seen that 40S and 60S of ribosome subunit peaks disappear from polysome fractionation?

I am doing polysome fractionation for stress conditions for mammalian cell. it seems that peaks of 40S and 60S disappear during stress condition compared to control. I am wondering whether all...

12 November 2018 3,513 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View