Yes absolutely ! Of course you need to use a metallic tip and a metallic substrate to close the circuit. see for example Article Local conductance measurement of graphene layer using conduc...
Also you can directly probe the moire effect when G is exfoliated on Boron nitride https://arxiv.org/pdf/1401.2637.pdf
Yes, but please remember that the contact resistance between the C-AFM tip and the sample and probably also the resistance of the set-up (tip+cable) are also measured because C-AFM is actually a two-contact electrical measurement. As a result, I think that while a comparison in term of conductivity can be done (assuming that the tip-sample contact is the same and the same tip is used), the measured value should not be claimed as the absolute value of the conductivity of the measured sample (unless a performed measurement is more elaborated than just scanning a C-AFM tip).