I would use DNA 1000 LabChips. I couldn't find articles suggesting the usage of this technology to analyse variation of alleles in populations.
During my master I tried to optimize a multiplex PCR. We didn't want to order expansive fluorescent labeled primers, since we were not sure the experiment would work.
Thus to see the different amplicon, I actually used the DNA 1000 LabOnChips. It worked fine, I could saw difference in fragment size up to 5bp if I remember correctly. You just have to be sure that your fragment won't be in the same range of size.
Hope it helps,
@ Barault and Siler: Minimum of 5 bp resolution may not detect the variation between (NN)8 and (NN)10. Capillary Electrophoresis is a good option.
Ramesh, I've done a lot of analyses on this device so far with some dominant, highly variable markers. Aside the claim from the specification sheet about the resolution of 5 bp, I have to say I've obtained highly comparable results with general sizing error of up to 2 bp. Furthermore, Lab-on-a-Chip is also based on capillary electrophoresis. In my research, I'm going to use 3, 5, and 6-nucleotide repeats. Will I improve confidence by putting an internal standard in, tracking its shift and consequent application to the loci peaks?
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