Hello,
I am interested in performing genomic DNA extractions and subsequent PCR analysis on some human cells (HEK293T). However, I am thinking of using a "colony PCR", i.e., by taking a number of cells and putting them into the PCR conditions and hoping that the initial denaturation temperature at 95℃ is enough to lyse the cells and release the genomic DNA.
Is this possible to be done? Has anyone attempted this, and if they have succeeded, how many cells are required and what are the parameters of the PCR conditions?
Thank you very much in advance!
Michael Gitonobel
You may make crude cell lysates and then conduct your genomic PCR. Just look for protocols for PCR genotyping and select one, which appears suitable for your experiments.
Arvind Kumar Shukla
Sorry, but you answer is not entirely correct.
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