Any good examples of mixed-methods studies with a qualitative focus ?

Hello, I'm looking for good examples of mixed-methods studies with a qualitative focus. I'm especially interested in the explanatory sequential design "case-selection" variant / preliminary...

13 June 2020 9,113 3 View

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Dear colleagues, In my experience, some evaluations received in the peer review process of journals, often include criticisms based on the QUANTITY of examples you can claim you have in a...

06 November 2019 8,055 4 View

A quick immunology question: Does this immunology graph seem correct?

Hi!! I have created a graph about the immune response in healthy and AD skin for S. aureus. Could I please ask if it should be a table or a figure? Does the pathway look correct? Shall I make it...

20 October 2019 1,706 3 View

Is there a "gold standard" for amino acid derivatization for LC/MS analysis?

We've recently started using a single quad LC/MS system (Shimadzu LC/MS-2020, for what it's worth) for mouse plasma AA analysis (the 20 common EAA and NEAA). Pre-column derivatization seems to be...

01 January 2019 1,398 5 View

50-50 dilution in elisa is better than no dilution?

Hi, I have been doing HBD-3 Elisa with highest sample concentration 50-50 dilution. I heard that 50-50 is better than adding 100uL of your sample, because you take into account the diluent as...

15 September 2018 3,118 3 View

Transformation efficiency in Corynebacterium glutamicum using GG ligation mix?

What steps can I take to increase the transformation efficiency of electroporated Cg? When transforming with a plasmid from E. coli, the efficiency is very high and I have ample colonies to choose...

25 July 2018 5,889 1 View

How to calculate technical efficiency in stata with a DEA and a big sample (27 cases, 5 inputs, 1 output)?

Hello, I am working on my master thesis in economics. I try to measure the technical efficiency of the European dairy sector. I understand Stochastic production frontiers and some DEA, but I...

23 May 2018 7,458 2 View

Has anyone identified the compound 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester in plant material?

I have found 3 citations where it has been extracted from plant material but I would need to know if someone else has found it since in my samples it is in a percentage higher than 20%

14 May 2018 480 3 View

De-salting methods without losing any proteins?

Hi, I am looking for methods of removing the salt from a solution, without using buffer exchange dialysis, or spinning down using membranes, so that no proteins are lost, no matter how small they...

28 March 2018 1,356 3 View

WB to detect Gft2h4, I don't have the band, but I have a perfect band on the control (H3), 4 different Ab tried and dot blot to, any suggestion about?

I'm doing WB to detect Gtf2h4 in mice's thymus and spleen. I used 4 different Ab from different companies and no one seems to work, but the positive control (histone 3) was perfect. I tried with...

19 October 2017 764 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View