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Questions related from Shraddha B Jogi
I am trying to integrate a 11 kb DNA, linearized with AvrII, at GAP locus in Pichia pastoris genome. I am using electroporation for the same. After transformation, I am observing colonies after...
28 May 2024 4,737 2 View
Is there any protocol available to transform a bacterial cell with two plasmid constructs?
19 February 2020 8,546 2 View
Hello all. So, we transformed RbCl E. coli DH5alpha cells using 50ng of DNA. But, we did not get a single colony. Can anyone explain the reason behind this problem? Note: RbCl comp cells,...
01 January 1970 7,569 3 View