Shraddha B Jogi

3 Questions 3 Answers 0 Followers

Questions related from Shraddha B Jogi

Is it possible to integrate >10 kb linear DNA into Pichia genome?

I am trying to integrate a 11 kb DNA, linearized with AvrII, at GAP locus in Pichia pastoris genome. I am using electroporation for the same. After transformation, I am observing colonies after...

28 May 2024 4,737 2 View

Is it possible to transform a single bacterial cell with two different plasmid constructs?

Is there any protocol available to transform a bacterial cell with two plasmid constructs?

19 February 2020 8,546 2 View

Unsuccessful transformation of Rubidium chloride E. coli DH5alpha cells using plasmid DNA

Hello all. So, we transformed RbCl E. coli DH5alpha cells using 50ng of DNA. But, we did not get a single colony. Can anyone explain the reason behind this problem? Note: RbCl comp cells,...

01 January 1970 7,569 3 View