Is it possible to identify saponin glycoside from nmr even if it hydrolysis during analysis and free sugars are present in the sample?

Samar M. Bassam Moaz @Samar-Moaz

02 March 2019 0 10K Report

I have isolated saponin glycoside after many efforts to get it in pure form; sephadex and prep tlc

However my saponins are very sensitive and they degrade giving me an glycoside and free sugars

I can purify again coz the amount is very small

Badges
Science topic

Similar topics
Political Science

More Samar M. Bassam Moaz's questions See All

How to prepare resistant cancer cells and how i keep it a life for along time?

Resistant cancer cells

17 May 2024 1,587 3 View

How to calculate IC50 for different populations have anticancer activity?

How to calculate IC50 for different populations have anticancer activity

26 April 2024 5,066 3 View

Is it ok to use a theoretical framework while conducting an IPA study?

I am conducting an interpretative phenomenological analysis, where I will be comparing the lived experiences of resilience among Syrian refugees residing in three different countries. Taking into...

08 January 2024 9,773 16 View

Any procedure for Synthesis of zinc chloride silica gel catalyst? And is it necessary to use anhydrous zinc chloride or we can use pure zinc chloride?

I want to synthesize few catalysts using silica gel.

18 October 2023 1,365 0 View

Can we use any catalyst to shorten the duration of synthesizing trolox ester?

I want to shorten the route of Synthesis of trolox ester via diels alder reaction.

18 October 2023 2,586 0 View

What is the accepted level of CO and NOx gas emission to the atmosphere ????

Greeting Researchers What is the accepted level of CO and NOx gas emission to the atmosphere ????

17 August 2023 5,521 2 View

Recommendations for Transcription Software?

I'm working on a qualitative study that will include 30 interviews conducted online in Arabic. Can you please recommend reliable transcription software that supports Arabic? It doesn't have to be free

27 July 2023 812 1 View

Can i carry out NMR analysis for swelling samples ?

i have synthesized a copolymer based on polyvinyl benzyl chloride and the resulting copolymer isnot soluble in DMSO or in any solvent but, instead it has a swelling character. Can i do NMR for...

20 July 2023 5,614 4 View

How to carry out biological tests like MTT or DPPH for insoluble samples ?

I need to evaluate the antioxidant and cytotoxicity activity for polymeric material . It doesnt dissolve in common organic solvents even dmso. Is there is a way to do such test!

27 June 2023 8,770 3 View

What is the research directions in battery thermal management?

I would like to use my expertise in CFD+Mechanical Engineering to advance research in thermal battery managment.

06 March 2023 4,613 1 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View