Hi, I am a research student working with expression of heterologous proteins in L. lactis, using the strictly Nisin controlled gene expression system (NICE®).
I know they can also replicate in E. coli, but require a recA+ strain like MC1061. A recA- strain, like DH5α, cannot be used for these vectors.
Manuel Zúñiga Cabrera
Hi,
This plasmid carries the pSH71 replication origin. It should replicate in most lactic acid bacteria and it can also replicate at least in Staphylococcus aureus and Bacillus subtilis.
You may want to have a look at this publication:
de Vos, W. M. 1987. Gene cloning and expression in lactic
streptococci. FEMS Microbiol. Rev. 46:281-295.
Hope this will help.
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