Is it okay if I aliquot the E8 medium+ supplement and freeze it down in -20 for long term?
Granular or powdered supplements usually do well at sub-zero temperatures as long as they are stored in a dry place in tightly closed containers. Liquids and pastes should be protected from freezing.
Thanks for your answer! Adam Popek
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
25 July 2024 3,395 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
18 July 2024 9,831 0 View
What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...
27 June 2024 2,138 2 View
in this link refractiveindex.info i didnt find i work on a simulation comsol of mesoporous perovskite i want to know the properties optoelectric and n,k coff
09 June 2024 2,071 4 View
Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!
11 May 2024 2,931 0 View
I had Titanium Dioxide powder & I need a specific answer for both points:- What is the most suitable solnavents used to convert TiO2 powder to TiO2 solution What is the most suitable methods...
30 April 2024 5,931 1 View
Hey, I am wondering which tests I can do to analyze my results and if there is a better way to analyze my results. If I have multiple treatments in columns and I have just one donor in a row,...
30 April 2024 335 2 View
Hey, Usually, when I perform total RNA extraction, I directly synthesize cDNA and run qPCR without even analyzing or testing the purity of RNA. I am asked to use 15µl of RNA when synthesizing...
20 March 2024 1,380 2 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
28 May 2024 1,398 0 View
Hi, I am currently creating a meta-analysis on the effect of growth performance of pigs in response to dietary fiber content in experimental diets as well as the influence of a fiber enzyme...
25 May 2024 8,855 1 View
I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...
12 May 2024 9,279 2 View
Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet
08 May 2024 9,891 1 View
05 May 2024 8,818 2 View
I am having a difficult time finding such a supplement, it's either discontinued by the manufacturer or for bovine or mice.
30 April 2024 6,993 0 View
Hi, I have been cultivating Jurkat cells in RPMI supplemented with 10% FBS, and I have observed some small things in my flask. Although the cell growth appears unaffected, I want to ensure that...
16 April 2024 5,466 3 View
Dear Colleagues, Have someone access to the Supplemental Material at http://link.aps.org/ supplemental/10.1103/PhysRevLett.120.265702 for Raman spectra and x-ray diffraction patterns at ambient...
11 April 2024 7,414 1 View
For neuronal media, there are some factors such as B27, N2, GDNF, and BDNF that are best added fresh into the basal media the day of. Instead of having a bunch of aliquots of each...
04 April 2024 8,733 2 View