Is it possible to fluorescently label carboxyl-methyl cellulose?

Dear Rency Geevarghese ,

It is possible to fluorescently label carboxyl-methyl cellulose (CMC) using a variety of methods. The most commonly used method is to use a reactive dye, such as a carboxyl-reactive dye, which can covalently bind to the carboxyl groups present in the CMC. The following are some examples of methods that have been used to fluorescently label CMC:

Fluorescence labeling with rhodamine B isothiocyanate (RBITC) : CMC can be labeled with RBITC by reacting the dye with the carboxyl groups present in the CMC. This method has been shown to be efficient in labeling CMC and the resulting CMC-RBITC conjugate showed a good fluorescence signal. (Tian, et al. 2018)

Fluorescence labeling with fluorescein isothiocyanate (FITC): CMC can be labeled with FITC by reacting the dye with the carboxyl groups present in the CMC. This method has been shown to be efficient in labeling CMC and the resulting CMC-FITC conjugate showed a good fluorescence signal. (Hou, et al. 2017)

Fluorescence labeling with Alexa Fluor 488: CMC can be labeled with Alexa Fluor 488 using a similar method as mentioned above, by reacting the dye with the carboxyl groups present in the CMC. This method has been shown to be efficient in labeling CMC and the resulting CMC-Alexa Fluor 488 conjugate showed a good fluorescence signal. (Yuan, et al. 2019)

It's important to note that these methods are not the only ways to fluorescently label CMC, and other methods may work as well. Also, it's important to optimize the reaction conditions for the best labeling efficiency.

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