I am trying to "see" GD2 and o-GD2 in TMA samples but all I find in literature is in fresh frozen tissue samples. Does someone have a protocol for FFPE samples?
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
03 March 2021 8,272 1 View
Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...
03 March 2021 2,037 3 View
Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).
03 March 2021 7,100 1 View
03 March 2021 5,360 2 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
02 March 2021 5,204 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View
Hello all, I've tried a Fluorojade-C stain according to the manufacturer's recommendations on FFPE tissue sections. My problem is that there is no staining, not even the DAPI control. I prepared...
11 January 2021 9,749 0 View
Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers
22 December 2020 599 3 View
Hello, I have a technical question for you. For RNA and DNA extraction, we use Quiagen kit with Trizol/thiocyanate de guanidine lysis. However, it's a bit long and we wanted to improve our...
14 December 2020 7,596 1 View
The FFPE tissues are relatively difficult to extract the RNA from so, how one can use reverse transcription based PCR
10 December 2020 4,398 6 View
Dear Collegues, If we want to perform immunohistochemistry /immunofluorence staining from FFPE human biopsy (vein) samples and study the presence of LPS and or common pathogen like Porphyromonas...
01 December 2020 5,744 2 View
Can anyone recommend a protocol to measure the telomerase activity in tissue samples? Before I was able to measure telomerase activity in cell populations using a telomerase activity measurement...
25 November 2020 1,509 1 View
I am trying to optimize TMA in my lab. When I start with 150 pg of DNA (Size 235 bp), I get good amplification, which I can visualize as an equivalent size dsDNA product, gel pic attached....
18 November 2020 7,622 3 View
The blade is set at a clearance angle of 5 degrees at which it has been sectioning very well. In the past few days with no change of blade type or type of wax. The blade has been cutting in too...
20 October 2020 769 5 View