27 July 2022 0 2K Report

I am trying to "see" GD2 and o-GD2 in TMA samples but all I find in literature is in fresh frozen tissue samples. Does someone have a protocol for FFPE samples?

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Is there a good protocol for FFPE fixation of defrozen PBMCs?

Hi all, I have been scouring the internet for a protocol for defrozen PBMC fixation and embedding. Currently I am defreezing PBMCs with an in-house protocol which gives me ~95% viability. After...

29 May 2024 7,463 2 View

Help! Alizarin Red not staining?

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Issue with DAPI staining on brain (dark spot in middle)?

Hi all, I have a question regarding something odd I'm seeing with DAPI on tissue. I'm trying to isolate the issue to prevent it in the future. I have sagittal brain sections on slides. For some...

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Cleaved Caspase-3 antibody staining for IHC not working on FFPE tissues?

Hello everyone, hope you are doing well. I have FFPE brain arteriovenous malformation (AVM) tissues I have been trying to stain with cleaved-caspase-3. I have used the Asp175 (D3E9) CC3 9579 from...

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Recommendation for pretreatment of pup mouse liver for FFPE tissue?

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Does anybody have good experience with Npas1 antibody on human post mortem brain tissue?

Hello! I want to try Npas1 staining on post mortem FFPE brain tissue, but I have no previous experience with Npas1 and am not sure which antibody to start with. If anybody has good experience with...

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How to quench/denature FAM, TdTomato in FFPE tissue sections?

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FFPE mouse femurs appear to have internal bubbles?

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How to Resolve DNA Extraction Failures in FFPE Tissue Processing?

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Have you ever digest keratin from FFPE tissues without compromising the quality of the tissues and RNA?

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