Yes it is possible. I recommend you lyse the erythrocytes and use Fc Receptors blocking antibodies before labeling the cells with conjugated antibodies. PBS (1x) supplemented with 2% of FBS can avoid unspecific cell staining.
Hi guys thanks for the answer.I hope you considered the fact that 100 micro litre of mice blood will have around 800 lymphocytes only.Flow cytometry normally requires around 20,000 events .I am attaching a protocol we have thought ,let me know if it will work.