I am trying to measure ATP content of my cells using Promega's CellTiter Glo, but then I would like to normalize to total protein. How do I go about this, without problems of interference from the CellTiter reagent?
So I want to see is the level of a secreted protein in the condition media via western blotting What my doubt is 1. How much cells should I seed and what should be the volume of condition...
19 June 2024 1,927 1 View
this electrode can't be open.
13 May 2024 2,991 3 View
I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....
16 April 2024 6,912 1 View
I want to prepare reduced form of NADPH. I am using 100mM of Tris (pH8.0) to make the buffer but the absorbance is showing to be 1.6 at 340nm which should supposed to be 0.6. can someone please...
11 March 2024 4,629 3 View
need a. Suggestion for best article related to management studies in data science
07 March 2024 3,506 1 View
Are there specific biochemical tests designed to analyze the ADMET properties of compounds, providing insights into their Absorption, Distribution, Metabolism, Excretion, and Toxicity?
25 December 2023 4,699 2 View
If you want to save time on manual referencing, I have posted 2 hr video on udemy. Mendeley desktop and web version helps us to store, organise and use reference as per any reference style. You...
28 September 2023 5,418 2 View
My reactants are not consumed and no new product spot is formed. My reaction conditions include dissolution of the acetophenone and aldehyde derivative in ethanol followed by dropwise addition of...
24 September 2023 1,908 5 View
To get better digestibility of the plant proteins (Fava bean protein isolates). What heat treatment should I give before the IVD (In vitro digestion) process ?
20 September 2023 7,124 0 View
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17 September 2023 4,135 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View