Dear Bhoj R Singh

Usually it's depend on the diagnosis methods Immunological and molecular tools, the number of replication of samples tested, ROMERO et al.,1995,mentioned no 100% confidence, article attached

Pls. find the attached files

http://www.nature.com/articles/ncomms1840

https://www.kncvtbc.org/en/what-we-do/strenghtening-laboratories-diagnostics/?gclid=CPGg8OzDzc8CFQIW0wodSMEIlw

http://www.oie.int/fileadmin/Home/eng/Media_Center/docs/pdf/Disease_cards/BOVINE-TB-EN.pdf

http://ftp.columbia.edu/itc/hs/pubhealth/p8442/lect02/MazurekJAMA.pdf

http://www.inspection.gc.ca/animals/terrestrial-animals/diseases/reportable/tuberculosis/fact-sheet/eng/1330208938232/1330209051950

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052851

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864480/

https://www.researchgate.net/publication/286936218_Adaptation_of_protocol_for_the_detection_of_bovine_TB_human_TB_and_para_TB_in_man_and_dairy_cattle_MZ_Hossain_UK_Rima_GA_Chowdhury_MA_Habib_ME_Haque_EH_Chowdhury_and_MAHNA_Khan

https://www.researchgate.net/profile/Leslie_Adams2/publication/12441026_A_multiplex_approach_to_molecular_detection_of_Brucella_abortus_andor_Mycobacterium_boris_infection_in_cattle/links/004635188472d01d9e000000.pdf

Hoping this will be helpful

Regards

Prof. Houda Kawas

Conference Paper Adaptation of protocol for the detection of bovine TB, human...

Article A Multiplex Approach to Molecular Detection of Brucella abor...

In biodiagnostics, there is rarely 100% sensitivity and specificity. Repeated sampling of milk, urine and stool (3 x each) and traditional culture of the material together with the clinical appearance of the animals will lead you to nearly 100% confidence.

sir, reg. tuberculosis in animals, as we know that it is sharing no. of antigenic determinants with other non tuberculosis mycobacteria which cross react in skin testing and gamma interferon assay..hence impairing the diagnosis. In addition, culture isolation and molecular diagnostic tools are also affected by internal and external factors related to samples collected for diagnosis from live animal. 

In total, we have to adopt a battery of tests to diagnose and control the disease. And strict compliance to control strategy is the bottom line of disease control and prevention.

Yes, I agree. So, key to success will be preparedness and alertness of farmers and/or veterinarians and/or authorities and collection of valid samples. The type of laboratory diagnosis (molecular, cultural) doesn´t make much of a difference.

With a single test it is extremely difficult  to diagnose animal tuberculosis in a dairy herd with 100% confidence. We need to find out a combination of tests that will increase the confidence level more towards 100%. I agree upon  the views put forwarded earlier. Our earlier experience, which showed tuberculin test followed by ante-mortem and post-mortem examinations to be effective in diagnosing tuberculosis in a dairy herd during 1990s. However, it required lot of formalities to follow the procedures. 

I fully agree with the previous comment.

Dear Werner Solbach

Any update of the diagnostic methods if not associated with a change in habits, methods and trading practice, this is not always through the issuance of decisions and even follow-up and adoption of several ministries, but the most important development of the means of warning and the media

Regards

Houda