Is it possible to develop a Rapid Diagnostics Test to know the infective (mosquito with sporozoit) malaria vector?

More Harishchandra Srivastava's questions See All

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Does plant based medicine have less side effects?

I was reading papers on plant secondary metabolites and their applications and many of the papers/books I read, have the point that plant-based medicines which are the result of application of...

08 July 2024 9,935 10 View

Hey everyone. I am facing some problems while transfecting A172 cell line with three plasmids for Dual Luciferase Assay. Any suggestions?

(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).

16 June 2024 6,687 2 View

Are the frequencies extracted from SdH oscillations nearly the same for different cemical substituted samples?

Generally, the chemical substitution should adjust the Fermi level, and the computed TSS indeed suggests that the Fermi pocket for the three samples different, so why are the frequencies extracted...

17 May 2024 1,897 1 View

Computed TSS seems to suggest that the sample should have stronger sdH oscillations, whereas experimentally extracted SdH oscillation is weak?

The sample nature is thin film

17 May 2024 8,459 0 View

How to define t shape gate structure in silvaco atlas?

I want to define T shape gate I added material air in free space , But it is showing boundry condition not defined electrode num=3 name=gate mat=gold x.min=2.6 x.max=3.5 y.min=0.03...

13 May 2024 3,059 0 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

13 May 2024 3,061 11 View

How to define t shape gate structure in silvaco atlas?

I want to define T shape gate I added material air in free space , But it is showing boundry condition not defined electrode num=3 name=gate mat=gold x.min=2.6 x.max=3.5 y.min=0...

13 May 2024 5,026 0 View

When can the fitting of the fluorescence anisotropy decays have the negative component ?

it would be helpful if you provide the reference to your answer

11 May 2024 5,989 4 View

What concentration of Nystatin should be used in Nutrient Agar medium?

I'm isolating bacterial from soil on Nutrient Agar media but after 4-5 days I see fungal growth start in the plates. I read about use of Nystatin to prevent fungal growth. How much quantity or...

21 April 2024 6,039 5 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

How are aPTT and PT values reduced after hemodialysis compared to before dialysis value in patients with chronic kidney disease?

I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...

30 July 2024 3,467 0 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

How do the complex dynamics of the oral microbiome interact with the host immune system affect periodontal disease development and treatment response?

This research question explores the complex dynamics of interactions between the oral microbiome and the host immune system, particularly in the development and treatment response of periodontal...

09 July 2024 7,922 1 View