pH of solutions is 6. Interday and intraday reproducibility is not possible. The same solutions, made from standard stock solution, have different absorbance values.
in what range of concentration are you measuring?
many articles says you can determine the Ethacridine lactate using HPLC in (pH 2.8) i am not sure if this pH is for the separation or the absorbance.
Dear Sir. Concerning your issue for the determination of Ethacridine lactate in water. It can be determined by UV spectrophotometer at 271 nm , But using HPLC research show that it is a pH dependant (pH 3) detected at 272 nm (Biomedical Chromatography, Volume 21, Issue 5, pages 480–483, May 2007) , you can visit the following link and the file attached which may help you in your analysis:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658061/
Thank
Dear Mirza:
Your compound can undergo keto-enol tautomerization because it has one group keto (that of the acid group) adjacent to one C bound to one -OH (-ol). This kind of compounds are extremely sensitive to pH. Although usually one -enol is one isomer of aldehydes or ketones, in your case you have one group -OH, and in the adjacent C one group ketone and another -OH. Anyway, in general the stability of the -C=O is greater than that of the ---C=C-. The enolization can be accomplished at acid or basic pH. For avoiding the enolization you should adjust the pH of your solution to 7, because in this case the enolization is very slow.
Best Regards,
Áurea Andrade Eiroa.
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