I've sequenced an exon from a particular gene of a candidate and from Forward-primer, chromatogram curve was detected to be interrupted while the result from reverse primer was detected to be readable (Agarose gel has given a single band; sequencing was repeatedly done for several times). Is this observation fair enough me to suspect for a possible whole exon deletion? Any explanation would be highly appreciated.
it sounds like a small insertion or deletion rather than an exon deletion in which case cloning and sequencing will confirm the sequences. If you post the electropherograms it would be easier to say with more confidence
Dear Herath,
I am also not sure what you mean by "interrupted" in forward primer. Did not cause it by homopolymer "only"? Anyway, a heterozygous deletion have to be seen in sequences from both directions.
Please post the sequence as Paul suggested if possible.
ok
Article Effect of KIT and PDGFRA Mutations on Survival in Patients W...
Dear Herath,
Please find our article in the attachments (ShiftDetector: detection of shift mutations
Seroussi et al., 2002). You simply can use in the public open software (http://cowry.agri.huji.ac.il) utilizing your one or two-directional electropherograms to determine size of deletion and sequence of both alleles.
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