I have been trying to find a way to produce a non-immunogenic variant of either HBsAg-S or HbC VLP carrier to express and demonstrate a protein of interest. I will use this VLP with antigen to detect memory B cells against the antigen in B-Cell ELISpot.
However, how can I be sure that the response is ONLY for the antigen on the VLP?
If I substitute the MIR-terminal or the antigenic determinant, can I be sure not to get an anti-VLP B-cell response then?
Thanks!
You can get certain valuable related informations by following the links below:-
1. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05204.x/pdf
2. http://www.google.com/patents/US8551756
I think this will be very difficult. Maybe as an alternative you could detect memory B cells by flow cytometry sorting. You would label the modified VLPs and wild-type VLPs with a fluor. Then, pre-screen (negatively select) the B cell population using wild-type VLPs, then positively select using the VLPs displaying the antigen of interest.
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