Depending on your purpose, there are some R and R/Bioconductor packages that can be used. Pathview is one that can be used with differential gene expression data, for instance. Cytoscape seems to be very popular for visualization so if you know how to use it, Nirmal's suggestion may be best.
My purpose is to reconstruct the pathway using the transcriptome data I have done annotation of my transcripts and identified some new enzymes which were not reported in the previous annotation now I need to construct the pathway will R programs suite my purpose
If it is transcriptome data such that you know log fold change values and which genes are differentially expressed, then Pathview will work. However Pathview will only work if you know the KEGG IDs for your genes/enzymes; if the new enzymes do not have a KEGG identity then they will not show up on the KEGG pathway.