Is it possible to check the transcriptional response of Wnt molecules in mice testicular cells?

10 October 2015 1 7K Report

I wanted to study Wnt transcriptional response in Mice Testicular cells. Please help me regards whether i can do this and which is the conventional method?

Petra Disterer

Dear Shashank,

if you mean with "transcriptional response" that you would like to see if Wnt expression is up or downregulated in certain mice versus other mice, then the accepted method is RT-qPCR, perhaps backed up with ELISA to determine protein levels.

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