I know from E500 brochure that Bruker has implemented this option in this spectrometer, but is it possible to apply this in other devices and if than how to do it?
I do not know this specific instrument, but working on usual epr spectrometers, i used a double resonant cavity to compare signal intensities with a permanent known sample (weak or strong pitch with know radical amounts. Then radiations can be used to create spins related to known radiation amounts that can be calibrated with alanine for example to determine the number of spins.
There is a good book about "quantitative EPR" (examples involve Bruker spectrometers, but they can be applied using any other spectrometer: organic radicals, free radicals induced by ionizing radiation etc.):
http://www.springer.com/us/book/9783211929476
However, if you can't access it, there is a free presentation:
https://portfolio.du.edu/downloadItem/220703
The rule of thumb is: use relative measurements as frequently as possible. You may introduce into a capillary tube a known amount of fresh DPPH and have it measured together with your sample. Care for the position is required.
Otherwise you may need to calculate the Q factor of the cavity with the sample introduced....and the list may grow pending on the desired degree of accuracy and on the details about your sample.
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