I know that THP-1 cells become adherent after exposure to PMA. Aside from this, are there any gross morphological characteristics that can be assessed by simple bright field microscopy? The vast majority of the cells attached to the substratum are ovoid in appearance, however there is a subset of the population that often exhibits two or more lateral processes, while some a almost completely flat with multiple processes. Is there a distinction between these cells? Does this morphological change become more pertinent after polarization of M0 to M1 and M2 as well? Conversely, is this merely representative of a more broad variability within the population?
https://www.researchgate.net/post/Why_do_THP-1_cells_adhere
https://www.researchgate.net/post/Culturing-THP-1-cells
Article Temporal dynamics and transcriptional control using single-c...
The above links may be helpful in understanding what is going on. Good luck.
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