It is possible, that's just a technique for finding the parameters. 

By routing, do you mean a (discrete) path problem?  In general, you rarely get the best results from applying a continuous technique (e.g. PSO) to a discrete/combinatorial problem or a discrete/combinatorial technique (e.g. ACO) to a continuous problem.

It is possible anyway. Firstly what are the numbers of parameters and how much levels for each one? if you have 4 parameters and 3 levels for each one then the perfect optimization is Box-Behnken Design (BBD).

You can use ARENA with suitable distribution function for simulation.  PSO is usually used in Multi-Objective Decision making problems to find optimal solutions.

ya sure... you Can use PSO for transport problem.

Hi, Kanticha Korsesthakarn,

Good Morning,

For your question:  Is it possible to apply Particle Swarm Optimization in simulations to optimize public transport systems.  For this, kindly go through the attachment PDF.

Kindly, go through the following publication.

Y. del Valle, G. Venayagamoorthy, S. Mohagheghi, J.-C. Hernandez, R. Harley, Particle swarm optimization: Basic concepts, variants and applications in power systems, IEEE Transactions on Evolutionary Computation, 12 (2) (2008) 171-195.

https://www.researchgate.net/publication/296636431_Codes_in_MATLAB_for_Particle_Swarm_Optimization?ev=prf_pub

I hope it will be very helpful to you.

Code Codes in MATLAB for Particle Swarm Optimization

Yes, here is PSO codes in MATLAB

https://www.researchgate.net/publication/296636431_Codes_in_MATLAB_for_Particle_Swarm_Optimization?ev=prf_pub

Code Codes in MATLAB for Particle Swarm Optimization

https://www.researchgate.net/publication/297245624_Particle_Swarm_Optimization_Algorithm_and_its_Codes_in_MATLAB

Research Particle Swarm Optimization: Algorithm and its Codes in MATLAB

https://www.researchgate.net/publication/280244004_A_comparative_study_of_metaheuristic_optimization_approaches_for_directional_overcurrent_relays_coordination

Article A comparative study of metaheuristic optimization approaches...

It does NOT make sense to "optimize" - yes, in quotes - a transit system based on the very unreliable methodologies that are discussed here. Metaheuristics should be banned from being applied in such an important infrastructure: (a) you have no idea how bad it can become, (b) there are already models that are more reliable, and (c) no-one would use the results as they are anyway.