This drugs have been used before, but only with local injections in the brain of rodents, which would require surgery, or, systemically in invertebrates. Therefore I am not sure systemic injections in rodents would be appropriate.
As part of a health lesson, I had my 4th grade students swab different areas of our classroom and plate them on agar plates. One student swabbed the seat of a wooden chair, and after 4 days of...
17 March 2024 8,044 0 View
We're trying to develop a more humane protocol for perfusion in laboratory mice. We had mice waking up during perfusion from anesthesia with avertin, but not with pentobarbital anesthesia using...
20 June 2021 5,524 4 View
Hello! I am trying to figure out how to run an analysis for a longitudinal moderated mediation. I will be collecting data twice from the same individuals in a mediation study while employing a...
12 May 2021 5,356 3 View
Hi, i am measuring translation efficiency of my target RNA-Protein Complex with RRL in vitro translation system (Promega) & Luciferase detection kit. It confused me that efficiency change...
08 December 2020 4,425 0 View
I am currently working on learning a cell dissociation protocol for adult mouse neural tissue (Miltenyi kit). The first time I ran through the protocol I was able to achieve something around 98%...
30 September 2020 6,550 0 View
This may be a really stupid question, and I have seen it done both ways. I am fairly new to mass spectrometry-based quantitation. I am trying to quantitate certain antibiotics (i.e. piperacillin...
02 June 2020 9,959 0 View
I have had a sudden die off of Zebrafish. All water quality parameters are optimal. I have also started treating for fungal and bacterial infection but I want to make sure I am on the right track....
07 April 2020 5,581 0 View
Hi Colleagues, I collected data for a project using survey monkey. I am in the process of cleaning the data for analysis, however, I have a large participant dropout issue. I know response...
08 March 2020 9,070 0 View
This is a research question for my English class that I will be doing. The question is based on how we can use a Therapeutic plan to treat mental disorders like Anxiety, Depression, Bipolar,...
24 February 2020 9,554 5 View
Hello - I have been attempting to macrophages via IF with F4/80 in OCT-embedded mouse skeletal muscle using Thermo Rat Anti-Mouse monoclonal Ab (Cat# MF48000) with little success. I have been...
20 December 2019 4,955 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View