Is it possible for a protein not to be detected at p-blast but show positive for its presence at Immunocytochemistry staining?

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Hala Hazzaa

I think the answer is "yes". Standard protein-protein BLAST (plastp) is used for both; identifying a query amino acid sequence and for finding similar sequences in protein database. Like other BLAST programs, blastp is designed to find local regions of similarity; but when the sequence similarity spans the whole sequence, blastp will report global alignment that is considered a preferred result for protein identification purposes.

Abdul Mannan Baig

Your reply is of great help.

Cheers Hala.

Have a great weekend.

Mannan

Welcome