High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How to solve the fatal error "the entire source was rejected" in MCNP?

Simple geometry was made having concentric cylinders bounded by a square region. The geometry has a reflective boundary. The source was defined at one point in the boundary and when it had been...

17 February 2021 4,364 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Hey guys, I'm looking for an efficient protocol to transfect patient-derived tumor cells with a high rate of transient transfection?

As you know it's quite tricky to get primary cells transfected at a relatively high rate!

31 January 2021 3,580 4 View

How can I model a vacuum region between a concentric tube in Ansys fluent?

I am modeling a double pipe (concentric tube) in Ansys Fluent. The center tube contains nanofluid, and the region between the pipes is a vacuum zone. The outer tube is used for reducing convection...

22 January 2021 2,781 3 View

What type of plants is better to work in gene regulation control project ?

I Interested in to work in gene network modeling thus I need to know what is future of gene network modeling in agriculture thanks

04 January 2021 6,527 3 View

Is it possible to culture several cell lines in the same plate/dish/flask with complete cell separation?

I'm trying to screen the effect of a toxic agent. I need to see its effect on a few different cell lines with different degrees of resistance to this compound. I would like to possibly culture...

31 December 2020 5,740 3 View

Help with insect identification?

Could someone help me identify the insect in this picture?

31 December 2020 2,109 5 View

How to find the natural frequency of nonlinear model (hyperelastic materials) in ansys APDL?

I'm working on the FE analysis of nonlinear vibration of circular plate in ansys APDL. I considered the Mooney-Rivlin model of hyperelastic materials. So i want to know that how to find natural...

27 December 2020 8,696 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View