No need of gel electrophoresis if you decided to go with Q RT PCR.

Based on CT value and delta delta CT method you should be able to see the difference in expression of your genes up on treatment . Unless you have problem with low expression of your gene of interest I think Q RT PCR method should work well .

Hi Romel,

You don't have to but I do run it at least once when I am establishing new primers.

However, if you have a positive control in your setup than you can just check the melting curves and analyze the whether the peaks in the curves are all the same or if you see also unspecific peaks or potentially primer dimers.

Hello Romel,

Of curse, there is no need to run products on the agarose gel to determine the expression levels, but just in case to make sure that amplificated target is exactly your GOI by checking the size of the band (as a confirm to melting curves) you can run your products on gel.

Best,

As Pranathi Meda mentioned, you don't need to run the products on a gel. You could, but you don't have to.

When setting up a new assay I always run the PCR products on a gel to confirm that I'm getting the appropriately sized amplicon. After that absolutely no gels.

you don't really have to, but double checking is always a good idea to confirm your results.

Thank you all for your answers.

After discussing it over with my supervisor, we have decided to run gel electrophoresis only for the first run of PCR.