Have anyone tried SYBR green with GoTaq DNA Polymerase for a qRT-PCR?

Hi, everyone. I need to perform a real time reverse-transcription PCR in one step. What we have is SYBR green I PCR Master mix with GoTaq DNA Polymerase. So far, our attemps to perform the assay...

07 January 2021 6,507 3 View

Does international survey based researches needs approval in each country ethics commitee?

We plan a contingent valuation of ecosystem services. The study will be based on surveys. We want to apply it in fifteen different countries. Is it necessary to approve the project in ethics...

25 June 2020 8,099 3 View

How to choose the best smooth algorithm for your data in Origin?

I have several curves of applied potential versus electric current with hundreds of points in each of them. I am trying to analyze their first and second derivatives, but they are too noisy to...

27 April 2020 5,932 2 View

I can't make my RAPD-PCR work anymore. Can anyone help me?

Guys, I REALLY NEED HELP At the end of the last year I optimized every parameter of my RAPD-PCR, and as a result I did obtain great products multiple times. Then, I went home for my vacation (I...

18 February 2020 9,236 3 View

No title

10 December 2019 7,354 5 View

Hi, I would like to solubilize a hidrophobic compound in order to administer it to mices. Should anyone suggest a nontoxic vehicle?

The compound is very insoluble in aqueous solution and the compound administration will be given orally. The compound manufacturer has no information regarding the solubility of the compound....

16 January 2019 834 1 View

No title

Hello everyone! I have been consulting this webpage http://beast.community/ and http://www.beast2.org/ , but the examples for the use of BEAUti and BEAST are for old versions. The versions of...

02 April 2018 3,942 3 View

Two-step cluster in SPSS - how to validate results?

Hi everybody. After applying a two-step cluster in SPSS, involving both continuous and nominal variables, how can I validate if the results are satisfactory? In other clustering methods there are...

03 October 2017 5,765 8 View

Have you COI sequences of Potamotrygon orbigyi?

24 August 2017 6,634 2 View

How many sequences it needed to put in a crown group to calibrate a tree?

17 August 2017 4,155 2 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View