In the protocol for the extraction of anthocyanins from plant material, the first step is the sample preparation. The sample is prepared by converting the plant material into a powdered form after mixing it with liquid nitrogen. Is it necessary to mix the plant material with liquid nitrogen or can we proceed without liquid nitrogen to convert plant material into powdered form?
The liquid N2 is to keep it cold, make it brittle, and exclude O2. The success otherwise depends on the properties of the particular material.
Kindly check https://www.intechopen.com/books/phenolic-compounds-natural-sources-importance-and-applications/anthocyanin-pigments-importance-sample-preparation-and-extraction
The liquid N2 is to keep it cold, make it brittle, and exclude O2. The success otherwise depends on the properties of the particular material.
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