Yes, but I think it is more important to estimate the number of cells when the inoculum reaches the late exponential phase, if you want to ensure a good number of cells, and it dependes on what you want to do.
This is very necessary to maintain the proportion equal. We have to give equal no. During the optimization processes so that there will be no error. If colonies no. Increase then also possibilities of increasing of enzyme activity. Therefore
Thank you to all for your answers. I estimated initially the biomass (dry weight) for this study and hence for each optimization experiment i have not done any bacterial count. Is it the right procedure i have performed?
Yes, by keeping the initial biomass same in every expt. we also optimize.
Yes it is necessary to avoid errors. it will give you correct information of best suited inoculum, concentration. and it is better not to widen the range in testing.
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