Some individual propose conventional PCR methods for molecular characterization. But it is qualitative in nature and less precise. What do you suggest?
Dear Yosef, on the base of my experience - conventional PCR is very useful tool in construction custom DNA sequences and other molecular manipulations in gene sequence editing (for example plasmide DNA). Moreover conventional PCR is crucial in the case of troubles with Real Time (first of all custom). Finally, agarose gel can provide a wide range of data for TaqMan or Sybr Green reaction optimization.
On the other hand Real Time is more "clean" in clinical trials and biomrdical research, I mean prevention of contamination from sample to sample. We don't need to open tubes after the reaction.
Of course, Real Time is fast and robust instrument that can provide quantitative information.
In conclusion I would say that both methods is useful but it depends on the Your goals.
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