Is it better to normalize Nile red absorbance to Bradford assay or to cell number?

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Anton Lennikov

I don't think any of the normalizations you described would make much sense. You are expected to seed an identical number of cells for such an experiment anyway. Unless you have concerns that your cell density is substantially different between the experimental and control groups. Otherwise, you can either present raw absorbance or normalize it to control by taking the absorbance average of your control group and dividing all the values by this number.