It's generally recommended to use a different set of barcode when reusing flow cells due to issues of barcode carry over despite thorough washing of the flow cell when running another batch of samples. Does that limitation still apply when using different sample types?, i.e. say first run, I'll sequence SARS-CoV2 amplicons using barcode set1, then say for the next sequencing run, I'll do a shotgun metagenomic approach for say bat intestine nucleic acid extracts using the same set of barcodes. And if I do that, are there bioinformatics approaches to discriminate the reads carry over from the first sequencing run?
Andrew Paul McKenzie Pegman
Yes! You can do whatever you like, there are no rules :)
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Kinesiology
- Running
More Michael G. Bacus's questions See All
Similar questions and discussions