Is it a problem if there is a PAM sequence in the gRNA?

More Çiğdem Yılmaz's questions See All

In the cerenkov.mac file of GEANT4 optical examples, can we change the scintillator and PMT material from LXe to NaI?

In the optical examples of GEANT4, specifically in the cerenkov.mac file where the LXe geometry is defined, is it possible to change the geometry and material of the scintillator and PMTs from LXe...

08 May 2024 7,236 0 View

Concentrating exosome for Western-Blotting?

Hello, I have isolated extracellular vesicles from a cell culture using the Exosome isolation kit. However, the western blot results did not show up because the concentration was only 0.2 ug/ul. I...

15 February 2024 8,378 4 View

(SSR marker) I used the same pair of primers and observed different band patterns in PCR. What is the reason for this?

In the SSR method, I used the same pair of primers (forward and reverse) in two different individuals, but I observed different band patterns in PCR.

05 February 2024 3,438 2 View

What is the reason for the changes in the band despite the use of the same SSR primer in both genotypes?

Genetic Engineering. Assuming that there were no errors during the process.

03 February 2024 2,832 0 View

After which processes can mRNAs obtained as a result of gene expression be sequenced?

Genetic Engineering. Sequence. mRNA sequence.

03 February 2024 1,375 2 View

What is the technical difference of CAPS and SCAR markers in genetic engineering?

Genetic Engineering. Markers.

03 February 2024 4,012 0 View

Why are 2 different restriction enzymes used when preparing genome libraries?

Genome library. Restriction Enzymes. Genetic engineering.

03 February 2024 5,429 2 View

Problems on Plant Based Protein Hydrolysis With Enzymes?

Dear All, I want to kindly ask a question about enzymatic hydrolysis of plant based proteins. We have proteases as Flavourzyme and we hydrolyze pea and wheat mixed protein. We construct a process...

01 October 2023 1,029 1 View

How can I detect different tones of the same colour that are very close to each other by image processing?

How can I detect different tones of the same colour that are very close to each other by image processing? I want to get the position of each tone in the flow.

13 August 2023 7,609 0 View

Why does DEA frontier find the efficiency of all DMUs 1?

I calculate the efficiency of public transportation vehicles via the DEA method and I use DEA frontier. It calculates the efficiency of all DMUs as 1. Can anyone identify what the problem is and...

05 June 2023 1,542 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View