Is it a Phytophthora species ?

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I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are?

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are? Is it possible that this microorganism is Stenotrophomonas panacihumi?

22 May 2024 875 4 View

What are the statistical approach for servant leadership in F&B?

To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?

01 March 2024 2,737 1 View

Do solar winds impact the spread of communicable disease on the earth?

We know that solar winds had a 11 year circle and also human respiratory diseases also outbroke every 11-12 year. Is there ant connection between solar wind's strength and the pandemic...

18 February 2024 6,280 5 View

In low doping level, i want to know why N-type threshold voltage has negative charge?

Basically, we know that the N-channel MOSFET has a (+) threshold voltage. However, the graph has a value of (-) depending on the acceptor doping concentration in the substrate and oxide capture...

24 January 2024 7,817 0 View

How to calculate the osmolality of Hepes solution?

Here is an excerpt from Kollmann et al. (2020, J. Physiol.): "This ring was placed in a recording chamber continuously perfused with 37°C aerated Hepes solution containing (in mM) 136 NaCl, 10...

09 January 2024 4,766 2 View

Heme in protein - MD simulation?

Dear all, I am currently working on a protein simulation with a heme group in the protein. I am wondering whether anyone can tell me how I can set it up in GROMCAS with charmm forcefile, since...

17 December 2023 4,392 2 View

Is it possible to use SPSS PROCESS MACRO(Model 1) to test moderation with categorical IV + binary DV + continuous Moderator?

IV: categorical - (5 categories with 1 and 5 being the extreme indicators) DV: binary - (dummy coding has been done.) Mo: continuous 1) Is it possible to use SPSS PROCESS MACRO for moderation...

08 November 2023 4,421 1 View

Western blot sample prep problem?

Hello, I have a question about western blot sample prep. First, I am using adherent cells and RIPA buffer. After centrifuge at 4 degrees Celsius, small pieces are still found when collecting the...

01 November 2023 7,135 0 View

Does the small intestinal manometry measure the gas pressure or liquid pressure?

Small intestinal pressure is one of the GI motility indicators and its manometry is very advanced nowadays. I wonder if there is any liquid inside the lumen during manometry. I already know the...

28 October 2023 2,491 1 View

How to combine trajectory in GROMACS when I repeat the same simulation for three times?

Hi all, I am wondering how I can combine the trajectory in GROMACS. I used the coding 'gmx trjcat -s xx.tpr -f xx-1.xtc xx-2.xtc xx-3.xtc -o xxx-combined.xtc' and 'gmx trjcat -s xx.tpr -f...

16 September 2023 2,539 0 View

I have NMR,Mass spec,IR and uv of a mixture of three unknown compounds from natural products.I am attaching the data who can help me elucidate?

I isolated a mixture of three compounds from the stem bark of a plant . I have NMR, Mass spec, IR, and UV data. There are a lot of things I can't make meaning out of. Kindly help me with the...

02 July 2024 9,531 4 View

What is the best part of a leaf to study the anatomy of monocot and dicot plants?

The first 1/3 of the leaf (near the stem), the middle 1/3 of the leaf, the last 1/3 of the leaf Monocot: Corn Dicot: bean

28 June 2024 2,607 4 View

Is it normal that buffer with 2% CTAB being so dense? It form two different phases when I mixed it with another buffer for DNA extraction ?

I am doing a DNA extraction from potato leaves infected with Phytophthora infestans for quantification via qPCR. The protocol includes two buffers: first, the tissue is disgragated with an...

19 June 2024 8,609 1 View

What is the best medium to isolate the Phytophthora erythroseptica?

Hi, I need your opinion on the best medium for cultivating Phytophthora erythroseptica, the fungus responsible for pink rot in potatoes. Additionally, I would like to know how to achieve maximum...

04 June 2024 8,864 3 View

What effect may biochar types produced from grape stems, leaves, onion leaves and stems, asparragus and cantaloupe stems/leaves have on a harvest?

I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...

28 May 2024 4,907 0 View

Is there a study of maize zein polymorphism using molecular markers?

Good afternoon. Please tell me, are there molecular markers of zeina genes for the purpose of genotyping lines and varieties? There are Asid-PAGE and SDS-PAGE methods for studying the...

25 May 2024 6,960 1 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Is it possible to have less than 1 % moisture content?

I am carrying out pharmacognostic analysis (physicochemical evaluation) of a stem bark of a ficus specie.

28 April 2024 6,130 0 View

What are the challenges faced by anaesthesiologists in the management of chronic pain, and how can these challenges be addressed?

Understanding Pain Physiology: Foundations for Effective Chr... What are the challenges faced by anaesthesiologists in the management of chronic pain, and how can these challenges be...

20 April 2024 3,314 1 View

How to convert ppm to micromoles per gram FW for Trehalose measurement from plants?

I would like to know how to convert ppm to micromoles per gram FW for trehalose measurement. I'm using the method described by by Li et al. (2014). Li, Z.-G., Luo, L.-J., & Zhu, L.-P. (2014)....

17 April 2024 4,038 2 View