Just wondering what is better to prepare sterile minimum media with labeled amino acids for SILAC experiments, autoclave or filtering. Autoclave would be fast but I don't know how deuterated tyrosine behaves there. On the other hand, filtering would be the optimal, but tyrosine is not super soluble to make a stock solution, and is also expensive.
Ideally, Deuterated compounds should be filtered. You could dissolve Tyrosine in 5M NaOH, then filter it with a syringe filter. Alternatively make the M9 medium with all components and use a Millipore Steritop unit to filter it. Filtering 1L Media with a Steritop Unit takes about 2min with water pump, I guess no autoclave can be faster then that!
Domenico is absolutely correct. Autoclaving uses steam (water) to sterilize and this will cause D2O - H2O exchange in your media.